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enzyme sphihf  (New England Biolabs)


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    Structured Review

    New England Biolabs enzyme sphihf
    Enzyme Sphihf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sphi+hf/SphI-HF/pm42097145-754-21-23
    Average 96 stars, based on 185 article reviews
    enzyme sphihf - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: A non-catalytic role for RFC in PCNA-mediated processive DNA synthesis
    Article Snippet: SphI-HF , New England Biolabs , Cat# R3182S.

    Article Title: Enhancer-promoter compatibility is mediated by the promoter-proximal region
    Article Snippet: PCR reactions were purified using NucleoSpin Gel and PCR Clean-up Kit (#11992242, Macherey-Nagel).

    Article Title: Enhancer-promoter compatibility is mediated by the promoter-proximal region
    Article Snippet: PCR products were gel purified and cloned into ClaI & SalI digested BMV1.1 donor plasmid backbone using HiFi DNA Assembly Master Mix (#E2621L, NEB) following manufacturer’s protocol.

    Control:

    Article Title: Treatment of a severe vascular disease using a bespoke CRISPR-Cas9 base editor in mice
    Article Snippet: .. The four control plasmids were cloned via ligation of annealed oligonucleotides ( Supplementary Table 4 ) into p11-lacY-wtx1 (Addgene ID 69056) digested with EcoRI-HF, SpeI-HF and SphI-HF (NEB). .. The HT-PAMDA substrate libraries containing the four control plasmids (combined at a ratio a 1:100 control plasmid pool to HT-PAMDA library) were linearized with PvuI-HF (NEB) before performing HT-PAMDA previously described 18 , 37 .

    Clone Assay:

    Article Title: Treatment of a severe vascular disease using a bespoke CRISPR-Cas9 base editor in mice
    Article Snippet: .. The four control plasmids were cloned via ligation of annealed oligonucleotides ( Supplementary Table 4 ) into p11-lacY-wtx1 (Addgene ID 69056) digested with EcoRI-HF, SpeI-HF and SphI-HF (NEB). .. The HT-PAMDA substrate libraries containing the four control plasmids (combined at a ratio a 1:100 control plasmid pool to HT-PAMDA library) were linearized with PvuI-HF (NEB) before performing HT-PAMDA previously described 18 , 37 .

    Ligation:

    Article Title: Treatment of a severe vascular disease using a bespoke CRISPR-Cas9 base editor in mice
    Article Snippet: .. The four control plasmids were cloned via ligation of annealed oligonucleotides ( Supplementary Table 4 ) into p11-lacY-wtx1 (Addgene ID 69056) digested with EcoRI-HF, SpeI-HF and SphI-HF (NEB). .. The HT-PAMDA substrate libraries containing the four control plasmids (combined at a ratio a 1:100 control plasmid pool to HT-PAMDA library) were linearized with PvuI-HF (NEB) before performing HT-PAMDA previously described 18 , 37 .

    Plasmid Preparation:

    Article Title: Protein-guided RNA barcoding links transcriptomes to synaptic architecture
    Article Snippet: .. The barcoded plasmid was generated by digesting the backbone pAAV-CAG-MVE-xrRNA-mScarlet-24xPP7 with XhoI and SphI-HF (New England Biolabs). .. The insert was amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs) with the WPRE_ssDNA_32bp_10Xbc forward primer and Sph1_reverse_primer_short (Supplementary Table 8).

    Generated:

    Article Title: Protein-guided RNA barcoding links transcriptomes to synaptic architecture
    Article Snippet: .. The barcoded plasmid was generated by digesting the backbone pAAV-CAG-MVE-xrRNA-mScarlet-24xPP7 with XhoI and SphI-HF (New England Biolabs). .. The insert was amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs) with the WPRE_ssDNA_32bp_10Xbc forward primer and Sph1_reverse_primer_short (Supplementary Table 8).



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